VP001
VirusExpress® 293T Lentiviral Production Cells
Pharma Manufacturing
Synonym(s):
HEK293T cells for lentivirus production
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About This Item
General description
VirusExpress 293T Lentiviral Production Cells are a clonal, suspension-adapted HEK293T derivative selected for fast growth, high transfection efficiency, and high-titer lentivirus production. VirusExpress 293T Lentiviral Production Cells grow to high cell densities, exhibit minimal cell clumping, and are amenable to PEI-based transfection. A medium exchange step is not needed prior to transfection with a PEI transfection reagent, and the cells can be transfected in larger volumes, enabling scale-up of lentivirus production. The cells are intended to be cultured in, and can be directly thawed into EX-CELL® CD HEK293 Viral Vector Medium.
VirusExpress 293T Lentiviral Production Cells are derived from the HEK293T cell line (ATCC CRL-3216) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham FL et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. HEK293T cells were created by stable transfection of the HEK293 cell line with a temperature-sensitive mutant of SV40 large T antigen. Due to the expression of the large T antigen, transfected plasmids that carry the SV40 origin of replication can replicate in 293T cells and will transiently maintain a high copy number. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs.
VirusExpress 293T Lentiviral Production Cells are derived from the HEK293T cell line (ATCC CRL-3216) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham FL et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. HEK293T cells were created by stable transfection of the HEK293 cell line with a temperature-sensitive mutant of SV40 large T antigen. Due to the expression of the large T antigen, transfected plasmids that carry the SV40 origin of replication can replicate in 293T cells and will transiently maintain a high copy number. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs.
Packaging
This product is shipped in a liquid nitrogen dry vapor shipper. The liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt.
Other Notes
The cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL. The cells were banked using under cGMP conditions in chemically defined medium supplement with 6 mM glutamine and 8% DMSO.
Legal Information
A commercial license must be taken prior to use of the cells, or any products or materials derived from the cells, in a manufacturing process. Please contact your local sales representative for more details.
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.
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