B8647

Brilliant Blue R Concentrate

suitable for SDS-PAGE, methanol solution

• Synonym(s): Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R
• Empirical Formula (Hill Notation): C₄₅H₄₄N₃NaO₇S₂
• CAS Number: 6104-59-2
• Molecular Weight: 825.97
• Colour Index Number: 42660
• Beilstein: 5718025
• MDL number: MFCD00041762
• UNSPSC Code: 12171500
• PubChem Substance ID: 24892085
• NACRES: NA.47

Properties

• form: methanol solution
• Quality Level: 200
• color: dark blue
• suitability: suitable for SDS-PAGE
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
• InChI: 1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
• InChI key: NKLPQNGYXWVELD-UHFFFAOYSA-M

Description

Application

Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining.
Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).

For detection of protein bands following electrophoresis.

Reconstitution

After dilution with deionized water to a final volume of one liter, solution will contain 0.25% (w/v) Brilliant Blue R, 40% (v/v) methanol and 7% (v/v) acetic acid.

Legal Information

Safety Information

• Pictograms: GHS02,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H225 - H301 + H311 + H331 - H315 - H319 - H370
• Precautionary Statements: P210 - P280 - P301 + P310 + P330 - P302 + P352 + P312 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1
• Target Organs: Eyes
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 57.2 °F - closed cup
• Flash Point(C): 14 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves