90922
Mueller Hinton Broth 2
NutriSelect® Plus, Cation-adjusted, suitable for microbiology
Synonym(s):
M-H 2 Broth, M-H 2 Broth, Mueller Hinton II Broth, Mueller Hinton II Broth
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About This Item
UNSPSC Code:
41171504
NACRES:
NA.85
Recommended Products
Quality Level
sterility
non-sterile
form
(homogeneous, hygroscopic powder)
shelf life
limited shelf life, expiry date on the label
composition
acid hydrolysate of casein, 17.5 g/L
beef extract, 3 g/L
starch, 1.5 g/L
packaging
bottle of 500 g
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbe id | susceptibility testing: suitable
microbiological culture: suitable
color
light yellow
final pH
7.3±0.2 (25 °C)
application(s)
clinical testing
food and beverages
microbiology
suitability
nonselective for Enterococcus spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Pseudomonas spp.
nonselective for Staphylococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms
Related Categories
General description
Mueller Hinton Broth 2 is recommended for testing the most encountered aerobic and facultative anaerobic bacteria found in food and clinical material for antimicrobial susceptibility testing. The medium shows good batch-to-batch reproducibility. It is low in sulfonamide, trimethoprim, and tetracycline inhibitors and yields satisfactory growth of most non-fastidious pathogens.Casein acid hydrolysate and beef extract provide amino acids and other nitrogenous substances, minerals, vitamins, and carbon to support the growth of microorganisms. To determine the MIC values with Enterococcus faecalis and sulfamethoxazole-trimethoprim, a special low content of thymine and thymidine in the amino acid sources is required. Starch neutralizes toxic substances that may be present in the medium and have an inhibitory effect on antibiotics and the growth of organisms. During autoclaving, a small amount of dextrose is hydrolyzed from starch, which is a source of energy. Calcium and magnesium salts are added to adjust the ion concentrations of the medium.
Application
Mueller Hinton Broth 2 cation adjusted is intended for use in quantitative procedures for susceptibility testing of rapidly growing aerobic and facultatively anaerobic bacteria isolated from clinical specimens. It has been used for culturing Escherichia coli, Bacillus subtilis, Staphylococcus aureus and in the serial dilution of doxycycline.
Features and Benefits
NutriSelect® Plus are powdered culture media offering
- Quality control with growth promotion test according to industry standards
- Quality assurance acc. to ISO 9001
- Cost-efficient powdered media
Preparation Note
Suspend 22 g in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense and sterilize by autoclaving at 10-15 lbs pressure (115-121°C) for 10 minutes. DO NOT OVERHEAT. Mix well before pouring.
Note: This medium is supplemented with appropriate salts to provide 20-25 mg/l of calcium and 10-12.5 mg/l of magnesium and as additionally required to suit performance parameters.
Note: This medium is supplemented with appropriate salts to provide 20-25 mg/l of calcium and 10-12.5 mg/l of magnesium and as additionally required to suit performance parameters.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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