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48900

Sigma-Aldrich

Giemsa Stain, Modified Solution

(for the staining (of cellular blood components and blood parasites))

Synonym(s):

Azure eosin methylene blue, Giemsa solution

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About This Item

CAS Number:
51811-82-6
EC Number:
200-659-6
MDL number:
MFCD00081642
UNSPSC Code:
41116121
PubChem Substance ID:
329757421
NACRES:
NA.25

type

(for research use only)

Quality Level

200

shelf life

limited shelf life

technique(s)

microbe id | staining: suitable

suitability

Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

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Application

Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.

Caution

store at 20°C

Principle

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Physical form

Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)

Reconstitution

Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

53.6 °F

Flash Point(C)

12 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG6252
BCCD3234
BCCB7813
BCBV9977
BCBS3921V

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Giemsa′s azur-eosin-methylene blue for microscopy

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