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215597

Sigma-Aldrich

Basic Fuchsin

Dye content >85 %

Synonym(s):

Basic Parafuchsin, Basic Red 9, Magenta™ O, Parafuchsin hydrochloride, Paramagenta hydrochloride, Pararosaniline chloride, Pararosaniline hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C19H17N3 · HCl
CAS Number:
569-61-9
Molecular Weight:
323.82
Colour Index Number:
42500
Beilstein:
4164603
EC Number:
209-321-2
MDL number:
MFCD00001657
UNSPSC Code:
12171500
PubChem Substance ID:
24852906
NACRES:
NA.47

Assay

>85.0% (HPLC)

Quality Level

100

form

solid

composition

Dye content, >85%

mp

268-270 °C (dec.) (lit.)

λmax

545 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=[NH2+])C=C3

InChI

1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H

InChI key

JUQPZRLQQYSMEQ-UHFFFAOYSA-N

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General description

Basic fuchsin is a triaminotriphenylmethane dye that is widely used to stain muscle, mitochondria, and collagen. It is generally a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520).

Application

Basic fuchsin is utilized for various applications:
  • Acid-fast staining
  • Periodic Acid-Schiff
  • Feulgen stain for DNA
  • ENDO culture medium
  • Leifson′s method for bacterial flagella

Biochem/physiol Actions

Basic fuchsin displays anesthetic, bactericidal (gram-positive), and fungicidal properties.

Legal Information

Magenta is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
SHBM2860
MKCH4239
MKBX6977V
MKBV6843V
80396HJV

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I A Schrijver et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 48(1), 95-103 (2000-02-01)
Integral immunohistochemical analysis of immune responses in frozen sections requires that, in addition to constitutively expressed membrane CD markers, less stable determinants can be reliably visualized. Therefore, we compared the commonly used acetone fixation method with pararosaniline fixation for six
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