MAHAS4510
Multiscreen® 96 well Plate, MCE membrane
pore size 0.45 μm, sterile
Synonym(s):
Detection Kit
About This Item
Recommended Products
material
flat bottom wells
mixed cellulose esters (MCE) membrane
polystyrene
Quality Level
description
Sterile, clear 96-well filter plate with 0.45 um pore size Mixed Cellulose Esters membrane for cell culture & ELISPOT Assays. Comes in a pack of 10.
sterility
sterile
product line
MultiScreen®
feature
lid
manufacturer/tradename
MultiScreen®
parameter
50-250 μL sample volume (per well)
technique(s)
DNA purification: suitable
ELISPOT: suitable
activity assay: suitable (with precipitations)
ligand binding assay: suitable
receptor binding assay: suitable
filtration area
0.28 cm2
plate size
96 wells
well maximum volume
300 μL
working volume
50-250 μL
matrix
MF-Millipore™
pore size
0.45 μm pore size
application(s)
sample preparation
binding type
high binding surface
shipped in
ambient
General description
HTS Filter Plates (Plate catalogue numbers that start with MS)
- MultiScreenHTS plates are specifically developed for high-throughput use with automated work stations. Rigid sidewalls improve handling and ample surfaces are provided for bar code labels. Wells are individually sealed to prevent incubation crosstalk and the underdrain is removable for access to the filters. Filter plates are available in 96- and 384-well platforms with a broad selection of membranes and plastics.
- Millipore’s new MultiScreen HTS+ Hi flow filter plate design lessens non-specific binding and reduces variability in both background and signal intensities specifically for biochemical screening assays.
Classic Filter Plates (Plate catalogue numbers that start with MA)
- The original multiwell design is valuable for many applications which require immersing the filter in an assay tray. The easily removable underdrain and unique plate design are ideal for assays such as Elispot, PAMPA, cell screening (migration, invasion or chemotaxis) or for topcount detection users.
Accessory – MultiScreenHTS Vacuum Manifold
- The MultiScreenHTS Vacuum manifold was designed specifically to work with MultiScreenHTS filter plates. The combination results in optimal filtration characteristics and seamless automation integration.
Millicell Assay Plates
- In addition to the traditional assay plate format, filter plates are also available with cell culture trays for growing and assaying suspension and adherent cell lines. Cells grown on filter plates differentiate better than those grown on traditional plastic substrates, resulting in more in vivo-like results This filter plate/tray combination can be used for a variety of cellular assays, including, transport, toxicity and screening assays.
Application
Cell Culture
Assays
Protein
Radionucleotides
Physical form
Other Notes
Legal Information
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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Articles
This article discusses attachment-dependent cell growth and cell proliferation investigations using the MultiScreen Assay System from Millipore.
The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.
Protocols
This page shows an example protocol of an ELISpot assay for quantifying the cells producing interferon-gamma in response to antigen or non-specific activation using phytohemagglutinin.
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