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Key Documents

MAB1383

Sigma-Aldrich

Anti-Integrin α4 Antibody, clone HP2/1

clone HP2/1, Chemicon®, from mouse

Synonym(s):

CD49d

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HP2/1, monoclonal

species reactivity

human, bovine, rat

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

NCBI accession no.

NM_000885.4

UniProt accession no.

P13612

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ITGA4(3676)
rat ... Itga4(311144)

Specificity

Recognizes the 80kDa alpha chain of VLA-4. Reacts with monocytes, T cells, B cells, thymocytes and Langerhans cells. Can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure. HP2/1 inhibits cell binding to soluble VCAM-1.

Immunogen

JM leukemia line

Application

Detect Integrin α4 using this Anti-Integrin α4 Antibody, clone HP2/1 validated for use in FC, IP, IH.
Flow cytometry: 1μg/5 X 10E5 cells

Immunohistology on frozen tissue sections

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Linkage

Replaces: 04-1131

Physical form

Format: Purified
Purified immunoglobulin - Ig fraction. Liquid in Phosphate Buffered Saline, pH 7.4 with 0.1% Sodium Azide.

Storage and Stability

Store at -20°C in undiluted aliquots. May be stored at 2-8°C for short term use. Avoid repeated freeze-thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Human eosinophil adherence to vascular endothelium mediated by binding to vascular cell adhesion molecule 1 and endothelial leukocyte adhesion molecule 1.
Weller, P F, et al.
Proceedings of the National Academy of Sciences of the USA, 88, 7430-7433 (1991)
Autotaxin, an ectoenzyme that produces lysophosphatidic acid, promotes the entry of lymphocytes into secondary lymphoid organs.
Hidenobu Kanda, Rebecca Newton, Russell Klein, Yuka Morita, Michael D Gunn, Steven D Rosen
Nature Immunology null
VLA-4 integrin on sarcoma cell lines recognizes endothelial VCAM-1. Differential regulation of the VLA-4 avidity on various sarcoma cell lines.
Mattila, P, et al.
International Journal of Cancer. Journal International Du Cancer, 52, 918-923 (1992)
VLA-3: a novel polypeptide association within the VLA molecular complex: cell distribution and biochemical characterization.
Sanchez-Madrid, F, et al.
European Journal of Immunology, 16, 1343-1349 (1986)
Jinah Park et al.
Oncogene, 23(19), 3474-3480 (2004-03-03)
Integrins are adhesion receptors that mediate both cell-extracellular matrix and cell-cell interactions. It has also been reported that the loss of integrin alpha4 expression might be associated with metastasis in several cancers. However, the molecular mechanism for loss of their
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