MAB10753

Anti-Caspase3 (active form) Antibody, clone 3D9.3

clone 3D9.3, from mouse

• Synonym(s): Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 3D9.3, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgMκ
• NCBI accession no.: NP_004337
• UniProt accession no.: P42574
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CASP3(836)

Description

General description

Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.

Specificity

This antibody demonstrates specificity for the cleaved 17 kDa active form, but does not cross-react with the non-active (pro) form Caspase3 at 35 kDa.

Immunogen

KLH-conjugated linear peptide corresponding to human Caspase3 (active form).

Application

Anti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Caspase3 (active form) in Hela and Hela/staurosporine 3 hr cells.
Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected Caspase3 (active form) in Jurkat T cells.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Caspase3 (active form) in 10 µg of HeLa and staurosporine treated HeLa cell lysate.

Target description

~17 kDa observed. Caspase3 is cleaved during apoptosis into 17 kDa and 12 kDa active forms.

Linkage

Replaces: 04-1090; 04-439

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable