DP02A
Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate
slurry (Liquid), clone PAb416, Calbiochem®
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
PAb416, monoclonal
form
slurry (Liquid)
contains
≤0.1% sodium azide as preservative
species reactivity
SV40-infected cells
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Supplied as an agarose conjugate. Recognizes the ~94 kDa SV40 large T antigen.
Recognizes the ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells. Cat. No. DP02A is linked to agarose for immunoprecipitation and purification.
This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate is validated for use in Immunoprecipitation, Affinity Purification for the detection of SV40 T Antigen (Ab-2).
Immunogen
Epitope: Within amino acids 83-128
purified SV40 large T-antigen
Application
Immunoprecipitation (15 µl suspension/sample)
Affinity Purification (see comments)
Affinity Purification (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
50% slurry in PBS.
Analysis Note
Positive Control
SV80 or SV-T2 cells
SV80 or SV-T2 cells
Other Notes
DeCaprio, J.A., et al. 1988. Cell54, 275.
Whyte, P., et al. 1988. Nature334, 124.
Mitchell, P.J., et al. 1987. Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.
Simanis, V. and Lane, D.P., 1985. Virol.144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology138, 379
Sarnow, P., et al. 1982. Cell28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.
Tooze, J.1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol.10,
Whyte, P., et al. 1988. Nature334, 124.
Mitchell, P.J., et al. 1987. Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.
Simanis, V. and Lane, D.P., 1985. Virol.144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology138, 379
Sarnow, P., et al. 1982. Cell28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.
Tooze, J.1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol.10,
Suitable for affinity purification and immunoprecipitation. The amount of suspension required for purification is dependent on individual sample types.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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