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AB2237

Sigma-Aldrich

Anti-PAX6 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Aniridia type II protein, paired box 6, paired box gene 6, paired box gene 6 (aniridia, keratitis), paired box homeotic gene-6

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, chicken

should not react with

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NM_013627

UniProt accession no.

P26367

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PAX6(5080)

General description

PAX6 is the most researched of the PAX genes and appears throughout the literature as a "master control" gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. This transcription factor is most famous for its use in the interspecifically induced expression of ectopic eyes and is of medical importance because heterozygous mutants produce a wide spectrum of ocular defects such as Aniridia in humans. This gene encodes paired box gene 6, one of many human homologues of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause aniridia as well as Peter′s anomaly, both ocular diseases.

Specificity

This antibody recognizes the C-Terminus of PAX6.

Immunogen

KLH conjugated followed by PAX6 corresponding to the C-Terminus

Application

Anti-PAX6 Antibody detects level of PAX6 & has been published & validated for use in WB & IC.
Immunocytochemistry:
Working dilutions of 1:50 - 1:1,000 of Anti-Pax6 was used to resolve the protein in chicken spinal cord.

Quality

Routinely evaluated by Western Blot on rat and mouse brain lysates.

Western Blot Analysis:
0.5 µg/mL of this lot detected PAX6 on 10 µg of mouse and rat brain lysates.

Target description

~48 kDa

Linkage

Replaces: AB5409

Physical form

Immunoaffinity purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Analysis Note

Control
Mouse and rat brain lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jovica Ninkovic et al.
Neuron, 68(4), 682-694 (2010-11-26)
Most neurons in the adult mammalian brain survive for the entire life of an individual. However, it is not known which transcriptional pathways regulate this survival in a healthy brain. Here, we identify a pathway regulating neuronal survival in a
Keith W Vance et al.
The EMBO journal, 33(4), 296-311 (2014-02-04)
Although some long noncoding RNAs (lncRNAs) have been shown to regulate gene expression in cis, it remains unclear whether lncRNAs can directly regulate transcription in trans by interacting with chromatin genome-wide independently of their sites of synthesis. Here, we describe
Notch signaling maintains neural rosette polarity.
Main, H; Radenkovic, J; Jin, SB; Lendahl, U; Andersson, ER
Testing null
Erin Knock et al.
Neural development, 10, 13-13 (2015-05-03)
Chromatin-modifying complexes have key roles in regulating various aspects of neural stem cell biology, including self-renewal and neurogenesis. The methyl binding domain 3/nucleosome remodelling and deacetylation (MBD3/NuRD) co-repressor complex facilitates lineage commitment of pluripotent cells in early mouse embryos and
Yury Kiselev et al.
Scientific reports, 8(1), 5059-5059 (2018-03-24)
Lung cancer is the leading cause of cancer deaths. Novel predictive biomarkers are needed to improve treatment selection and more accurate prognostication. PAX6 is a transcription factor with a proposed tumour suppressor function. Immunohistochemical staining was performed on tissue microarrays
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