71137

Rosetta-gami B(DE3)pLysS Competent Cells - Novagen

Rosetta-gami B strains combines the key features of BL21, Origami, and Rosetta to enhances the expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

• UNSPSC Code: 41106202

Properties

• biological source: Escherichia coli
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: transformation efficiency: 1.0×10⁶ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Genotype: F^–ompT hsdS_B (r_B^– m_B^–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysSRARE (Cam^R, Kan^R, Tet^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Rosetta-gami B strains combine the key features of BL21 (and its Tuner derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Rosetta-gami B strains combines the key features of BL21, Origami, and Rosetta to enhances the expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta-gami B(DE3)pLysS Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids