1.10866

GranuCult^® plus WL nutrient agar

for yeasts, suitable for microbiology

• Synonym(s): Wallerstein Laboratories Nutrient Agar
• UNSPSC Code: 41106213

Properties

• form: medium granules (dehydrated (DCM))
• Quality Level: 200
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• storage condition: dry media (Keep Tightly closed)
• technique(s): microbiological culture: suitable
• color: blue-green
• pH: 5.3-5.7 (25 °C, 77 g/L in H₂O, after autoclaving)
• solubility: 77 g/L
• bulk density: 730 kg/m³
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: yeasts

Description

General description

GranuCult^® plus WL (Wallerstein Laboratories) Nutrient Agar has a pH of 5.5, which is optimal for the enumeration of brewers′ yeast. If bakers′ or distillers′ yeast is to be examined, the pH should be adjusted to 6.5 (better yields). When cultivating microorganisms from an alcoholic mash, tomato juice should be added to the medium. WL differential agar contains cycloheximide to suppress yeasts and any molds that may be present; this medium allows reliable counting of all bacteria that may be encountered in the tests performed in brewery laboratories.

Application

GranuCult^® plus WL nutrient agar is suitable for the enumeration and cultivation of yeasts and bacteria in the microbiological control conducted in the brewing and other fermentation industries.

Features and Benefits

• GranuCult^® offers superior granulated culture media.
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions.
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): blue-green
pH-value (25 °C): 5.3 - 5.7
Growth (Candida albicans ATCC 10231 (WDCM 00054)): good to very good
Growth (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): good to very good
Growth (Lactobacillus acidophilus ATCC 4356 (WDCM 00098)): fair to very good
Growth (Leuconostoc mesenteroides ATCC 9135 (WDCM 00108)): good to very good
Growth (Enterococcus faecalis ATCC 11700): good to very good
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): good to very good
Growth (Proteus mirabilis ATCC 29906 (WDCM 00023)): good to very good
Incubation: 4 days; 28 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3