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Merck
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1.02137

Millipore

Contact E

Envirocheck™, for enterobacteriaceae

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About This Item

UNSPSC Code:
41116116
NACRES:
NA.74

material

plastic cap
plastic slide
plastic tube

Quality Level

100

product line

Envirocheck™

form

solid (ready-to-use)

manufacturer/tradename

Envirocheck™

pH

6.8-7.5 (25 °C in H2O)

application(s)

food and beverages
surface monitoring
water monitoring

storage temp.

2-30°C

suitability

enterobacteriaceae

General description

Envirocheck Contact Enterobacteriaceae, a semi-quantitative detection of microorganisms on surfaces and in liquids, are flexible paddles that are coated with Tryptic soy agar to detect the bacteria belonging to the family Enterobacteriaceae from the samples. Slide A is coated with Plate Count Agar to obtain the total viable (aerobic) count. Slide B is coated with Violet Red Bile Dextrose (VRBD) Agar, recommended for the isolation and enumeration of all Enterobacteriaceae species in foodstuffs.

Application

This medium is recommended for the selective isolation and enumeration of all Enterobacteriaceae species in foodstuffs. Envirocheck contact slides can be used for both surface and liquid testing in industrial and environmental monitoring.

Analysis Note

Count the number of colonies on both sides of the paddle. Plate Count Agar: Count the number of colonies for obtaining the total aerobic bacterial count (TVC). Violet Red Bile Dextrose (VRBD) Agar: Degradation of dextrose is accompanied by production of acid which is indicated by a colour change to red and by zones of precipitated bile acids surrounding the colonies.
Sterility: passes test
pH-value/side one (25 °C): 6.7 - 7.3
pH-value/side two (25 °C): 7.0 - 7.6
Growth/side one
- Escherichia coli ATCC 8739 (WDCM 00012):
good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): good
- Salmonella typhimurium ATCC 14028 (WDCM 00031): good
- Shigella sonnei ATCC 25931: good
Growth/side two
- Escherichia coli ATCC 8739 (WDCM 00012):
fair/good
- Staphylococcus aureus ATCC 6538 (WDCM 00032): none
- Salmonella typhimurium ATCC 14028 (WDCM 00031): fair/good
- Shigella sonnei ATCC 25931: fair/good
Incubation: 48 hrs.; 35 °C; aerobic.

Legal Information

ENVIROCHECK is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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