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G006124

HPLC Analysis of Salicylic Acid and Acetylsalicylic Acid (Aspirin) on Ascentis® Express OH5

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2.7 μm particles (53769-U)
mobile phase: 2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 7.0 with acetic acid, glacial
flow rate: 0.5 mL/min
pressure: 850 psi (59 bar)
column temp.: 35 °C
detector: UV, 235 nm
injection: 2 μL
sample: 100 μg/mL salicylic acid, 200 μg/mL acetylsalicylic acid in methanol:acetonitrile (3:7)

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Salicylic acid, meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (calc. to the dried substance)
    Acetylsalicylic acid, purum, ≥99.0% (HPLC)

analytical column

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