G005881

LC/MS/MS Analysis of Drugs of Abuse: Generic Screening on Ascentis^® Express F5

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U); mobile phase: [A] 5 mM ammonium formate water pH 4.0 formic acid; [B] 5 mM ammonium formate (95:5 acetonitrile:water); gradient: 20% B for 0.5 min; to % B in 5.5 min; held at 100% B for 3 min; flow rate: 0.3 mL/min; pressure: 2538 psi (175 bar); column temp.: 35 °C; detector: MS, ESI(+), TIC, m/z 100-1000; injection: 1 μL; sample: each compound 1000 ng/mL in 40:60 (water:methanol)
• suitability: application for HPLC
• application(s): forensics and toxicology

Description

Analysis Note

Shown here is a method to rapidly screen a sample for a variety of abused substances using an Ascentis Expres F5 column. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Benzoylecgonine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Morphine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Codeine solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Oxycodone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Normeperidine solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Hydrocodone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
(±)-MDMA solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
(±)-Methamphetamine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Meperidine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Fentanyl solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
(+)-Propoxyphene solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant^®
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