819695

TSKgel^® Amide-80 HPLC Column

phase carbamoyl, L × I.D. 10 cm × 2 mm, 5 μm particle size

• Synonym(s): TSKgel^® Amide-80
• UNSPSC Code: 41115711
• eCl@ss: 32110501

Properties

• material: stainless steel hardware
• Agency: suitable for USP L68
• product line: TSKgel^®
• manufacturer/tradename: Tosoh 19695
• parameter: 0-100% organic solvent; 10-80 °C temperature; 80 bar max. pressure
• technique(s): HPLC: suitable
• L × I.D.: 10 cm × 2 mm
• surface area: 450 m²/g
• matrix: silica particle platform
• matrix active group: carbamoyl phase
• particle size: 5 μm
• pore size: 100 Å
• operating pH: 2.0-7.5
• separation technique: hydrophilic interaction (HILIC)

Description

General description

TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation