58338

SUPELCOSIL^™ LC-NH₂ HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

• EC Number: 276-161-8
• UNSPSC Code: 41115700
• eCl@ss: 32110501

Properties

• Agency: suitable for USP L8
• Quality Level: 100
• feature: endcapped
• manufacturer/tradename: SUPELCOSIL^™
• extent of labeling: 3% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable
• L × I.D.: 25 cm × 4.6 mm
• surface area: 170 m²/g
• surface coverage: surface coverage 5.1 μmol/m²
• matrix: silica gel, spherical particle platform
• matrix active group: amino phase
• particle size: 5 μm
• pore size: 120 Å
• application(s): food and beverages
• separation technique: hydrophilic interaction (HILIC); normal phase

Description

General description

Silica-based SUPELCOSIL LC-NH2 columns separate monosaccharides, disaccharides, and some trisaccharides, with elution generally in order of increasing molecular weight. Retention decreases as the proportion of water:acetonitrile in the mobile phase is increased. For more information, request T397126 (Sugars on SUPELCOSIL LC-NH2 Columns).

The amino column is most often employed for the separation of mono- and disaccharides. As a normal-phase application, amino columns are used in the petroleum industry (see SUPELCOSIL^™ LC-NH₂-NP HPLC Columns for additional details).

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC