58220-U
SUPELCOSIL™ LC-8 (5 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Synonym(s):
LC-8 Reverse-Phase HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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product name
SUPELCOSIL™ LC-8 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Agency
suitable for USP L7
product line
SUPELCOSIL™
feature
endcapped
packaging
1 ea of
extent of labeling
6.0% carbon loading
parameter
≤70 °C temp. range
technique(s)
HPLC: suitable
L × I.D.
15 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 3.2 μmol/m2
matrix
silica gel, spherical particle platform
fully porous particle
matrix active group
C8 (octyl) phase
particle size
5 μm
pore size
120 Å
pH range
2-7.5
application(s)
food and beverages
separation technique
reversed phase
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General description
A phase less hydrophobic than C18. Provides less retention of both polar and non-polar compounds than C18. Use a mobile phase containing 5% less organic modifier for the C8 column than C18. Polar compounds are, relatively, more strongly retained on C8 than C18 columns.
Application
- Chromatographic behaviour of ionic liquid cations in view of quantitative structure-retention relationship.: This study explores the chromatographic behavior of ionic liquid cations using quantitative structure-retention relationships (QSRR). The SUPELCOSIL™ LC-8 HPLC Column was utilized to analyze retention data for various ionic liquids, providing insights into their separation mechanisms and potential applications in analytical chemistry (Molíková et al., 2010).
- Liquid chromatographic method for the determination of lidocaine and monoethylglycine xylidide in human serum containing various concentrations of bilirubin for the assessment of liver function.: The research presents a liquid chromatographic method using the SUPELCOSIL™ LC-8 HPLC Column to determine lidocaine and its metabolite in human serum. This method is particularly useful for assessing liver function by measuring drug metabolism (Piwowarska et al., 2004).
- LC determination of morphine and morphine glucuronides in human plasma by coulometric and UV detection.: The research describes a method for the determination of morphine and its glucuronides in human plasma. Utilizing the SUPELCOSIL™ LC-8 HPLC Column, the study demonstrates the method′s sensitivity and applicability in pharmaceutical analysis (Ary et al., 2001).
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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F Belal et al.
Journal of pharmaceutical and biomedical analysis, 24(3), 335-342 (2001-02-24)
A simple, rapid and sensitive HPLC method has been developed for the simultaneous determination of ramipril and hydrochlorothiazide in their dosage forms. Acetonitrile: sodium perchlorate solution (0.1 M) adjusted to pH 2.5+/-0.2 with phosphoric acid (46:54 v/v), was used as
Thomas Borch et al.
Journal of chromatography. A, 1022(1-2), 83-94 (2004-02-03)
Explosives such as 2,4,6-trinitrotoluene (TNT), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) are widely distributed environmental contaminants. Complete chromatographic separation is necessary in order to accurately determine and quantify explosives and their degradation products in environmental samples and in (bio)transformation studies. The
Jadwiga Piwowarska et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
S B Ruddy et al.
Journal of chromatography. B, Biomedical applications, 657(1), 83-92 (1994-07-01)
The isocratic, reversed-phase, high-performance liquid chromatographic (HPLC) method presented provides a simple and rapid analytical technique for the simultaneous determination of low-molecular-mass oligomers of polyethylene glycol (PEG) in aqueous polymer samples containing polar contaminants of biologic origin. In the present
K Ary et al.
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium
Protocols
Protocol for HPLC Analysis of Phenols on SUPELCOSIL™ LC-8
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