ZRB2161

Anti-MEX3A Antibody, clone 1J23 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): RNA-binding protein MEX3A;RING finger and KH domain-containing protein 4
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1J23, recombinant monoclonal
• description: 1J23 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 54.17 kDa; observed mol wt ~54 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): porcine, bovine, monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_001087194
• UniProt accession no.: A1L020
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... MEX3A(92312)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1J23 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects RNA-binding protein MEX3A. It targets an epitope within 16 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human RNA-binding protein MEX3A.

Application

Quality Control Testing

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MEX3A in SH-SY5Y cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MEX3A in Neuro2A and U87MG cell lysates.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MEX3A in SH-SY5Y cells.

Affinity Binding Assay: A representative lot of this antibody bound MEX3A peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

RNA-binding protein MEX3A (UniProt: A1L020; also known as RING finger and KH domain-containing protein 4) is encoded by the MEX3A (also known as RKHD4) gene (Gene ID: 92312) in human. MEX3A is a member of the MEX3 family that widely exists in mammalian cells and encodes four different genes, MEX3A, MEX3B, MEX3C, and MEX3D. These four genes are located on distinct chromosomes and encode closely related proteins. MEX3A is an RNA-binding phosphoprotein that is involved in post-transcriptional regulatory mechanisms. It is predominantly expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway. Its highest levels are observed in fetal brain and testis. Its expression is also observed in thymus, salivary gland, and uterus. MEX3A contains two RNA-binding KH domains (aa 132-193 and223-284) and a ring domain with E3 ubiquitin ligase activity at the C terminal. The ring finger domain provides MEX3 protein the ability to regulate ubiquitination of target proteins and influencing their protein stability and subcellular localization. Overexpression of MEX3A is reported in gliomas and is linked to higher malignancy and poor prognosis. In vitro loss-of-function and gain-of-function studies show that MEX3A promotes glioma development through regulating cell proliferation, cell apoptosis, cell cycle, and cell migration. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Yang, C., et al. (2021). Cell Death Dis. 12(1); 14; Buchet-Poyau, K., et al. , (2007). Nucleic Acids Res.35(4); 1289-1300).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable