ZRB1881

Anti-MCT1/SLC16A1 Antibody, clone 2B21 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Monocarboxylate transporter 1;Solute carrier family 16 member 1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2B21, recombinant monoclonal
• description: 2B21 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 53.94 kDa; observed mol wt ~54 kDa
• purified by: using Protein A
• species reactivity: rat, human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: NP_001159968
• UniProt accession no.: P53985
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... SLC16A1(6566)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2B21 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Monocarboxylate transporter 1 (MCT 1). It targets an epitope within 15 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Monocarboxylate transporter 1 (MCT 1).

Application

Quality Control Testing

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected MCT1/SLC16A1 in L6 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MCT1/SLC16A1 in lysates from Rat skeletal muscle tissue and U-251 MG cells.

Flow Cytometry Analysis: 1 μg from a representative lot detected MCT1/SLC16A1 in one million U-251 MG cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MCT1/SLC16A1 in U-251 MG cells.

Affinity Binding Assay: A representative lot of this antibody bound MCT1/SLC16A1 peptide with a KD of 1.7 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected MCT1/SLC16A1 in L6 cell lysate.

Target description

Monocarboxylate transporter 1 (UniProt: P53985; also known as MCT 1, Solute carrier family 16 member 1) is encoded by the SLC16A1 (also known as MCT1) gene (Gene ID: 6566) in human. MCT1 is a widely-distributed, multi-pass transmembrane protein with seven cytoplasmic domains, twelve transmembrane domains, and six extracellular domains. It serves as a proton-coupled monocarboxylate transporter that facilitates rapid transport of lactate, pyruvate, branched-chain oxo acids, and ketone bodies. It is required for normal nutrient assimilation, increase of white adipose tissue, and body weight gain when on a high-fat diet. It plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, and glucose homeostasis. Mutations in SLC16A1 gene are known to cause monocarboxylate transporter 1 deficiency disease (MCT1D) that is characterized by recurrent ketoacidosis. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Martini, T., et al. (2021). Front. Physiol. 12:665476; van Hassel, PM., et al. (2014). N. Engl. J. Med. 371(20); 1900-1907).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable