S9012

Sulforhodamine B sodium salt

Technical grade

• Synonym(s): Acid red 52; Acid Rhodamine B
• Empirical Formula (Hill Notation): C₂₇H₂₉N₂NaO₇S₂
• CAS Number: 3520-42-1
• Molecular Weight: 580.65
• Colour Index Number: 45100
• Beilstein: 3886008
• EC Number: 222-529-8
• MDL number: MFCD00010180
• UNSPSC Code: 12171500
• PubChem Substance ID: 24899824
• NACRES: NA.47

Properties

• Quality Level: 200
• form: powder
• composition: Dye content, ~75%
• εmax: ≥85000 at 554-560 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1
• InChI: 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
• InChI key: SXQCTESRRZBPHJ-UHFFFAOYSA-M

Description

General description

Sulforhodamine B (also known as SRB, kiton rhodamine B, lissamine rhodamine B, or sulphorhodamine B) is a bright-pink anionic, aminoxanthene dye carrying two sulfonate substituents.

Application

Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.

It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.

It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves