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RAB0245

Sigma-Aldrich

Mouse IL-10 ELISA Kit

for serum, plasma and cell culture supernatant

Synonym(s):

IL-10, Interleukin-10

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 45 pg/mL
standard curve range: 78.13-5000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Il10(16153)

Related Categories

General description

The Mouse IL-10 (Interleukin-10) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse IL-10

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

IL-10 (interleukin 10) is an anti-inflammatory cytokine. In mouse, IL-10 produced by the CD4+ Th2 (T helper cell 2) subset, inhibits the expression of cytokines specifically IFN-3 (interferon), by Thl (T helper cell 1) clones. mIL-10 has pleiotropic property and it interacts with various cell types such as thymocytes, cytotoxic T cells, mast cells, B cells, and macrophages. Polymorphisms in the IL-10 gene are associated with higher risk of preeclampsia, ankylosing spondylitis, nasopharyngeal carcinoma and basal cell carcinoma. B- cell specific IL-10 might have a suppressive role in systemic lupus erythematosus (SLE).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABELADDELISA 5X Assay/Sample Diluent Buffer D (Item K)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Clinical significance of IL-2 and IL-10 gene polymorphisms and serum levels in patients with basal-cell carcinoma.
Sobjanek M
Biomarkers in Medicine, 10(2), 185-195 (2016)
Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes.
de Waal Malefyt R
The Journal of Experimental Medicine, 174(5), 1209-1220 (1991)
The Influence of Bone Marrow-Secreted IL-10 in a Mouse Model of Cerulein-Induced Pancreatic Fibrosis.
Lin WR
BioMed Research International (2016)
Investigations into the association between polymorphisms in the interleukin-10 gene and risk of early-onset preeclampsia.
Liu QY
Genetics and molecular research : GMR, 14(4), 19323-19328 (2015)
IL-10 Production Is Critical for Sustaining the Expansion of CD5+ B and NKT Cells and Restraining Autoantibody Production in Congenic Lupus-Prone Mice.
Baglaenko Y
PLoS ONE, 11(3), e0150515-e0150515 (2016)
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