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R8005

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RPMI-1640 Medium

Hybri-Max™, Modified, with L-glutamine, 4500 mg/L glucose and 15mM HEPES, without sodium bicarbonate, powder, suitable for hybridoma

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Synonym(s):

RPMI 1640 Culture Medium

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About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
NaHCO3: no
glucose: 4.5 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Quantity

Formulated to contain 16.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michael D Lewis et al.
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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