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P7059

Sigma-Aldrich

Phosphate Buffered Saline

10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology

Synonym(s):

Phosphate buffered saline, PBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate Buffered Saline, 10× PBS for Western blots and IP

grade

for molecular biology

sterility

0.2 μm filtered

form

liquid

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

pH

7.2-7.6(1x solution)

storage temp.

room temp

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General description

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Application

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Components

10x PBS contains:
  • 30mM sodium phosphate
  • 1.5 M sodium chloride
  • 10.5 mM potassium phosphate

Preparation Note

Dilute 10x PBS to a 1x working solution using molecular biology grade water.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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