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NA1111

Sigma-Aldrich

GenElute™ Gel Extraction Kit

sufficient for 70 purifications

Synonym(s):

DNA gel extraction kit, DNA gel isolation kit, DNA gel purification kit, PCR gel clean up kit, PCR gel isolation kit, gel isolation kit, gel purification kit, Rapid purification of linear DNA

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

usage

sufficient for 70 purifications

technique(s)

DNA purification: suitable

storage temp.

15-25°C

General description

The GenElute Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.

Application

GenElute™ Gel Extraction Kit has been used for the purification of DNA fragments from agarose gels.
The isolated DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.

Features and Benefits

  • Bind up to 10 μg of DNA
  • Recoveries up to 80%
  • Up to 3.5 g can be processed per column
  • Compatible with both standard and low-melting agarose in TAE or TBE buffer

Principle

The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted by solubilizing slices of an agarose gel. The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer. The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.
The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane. Contaminants are removed by a simple spin or vacuum wash. The bound DNA is then eluted.

The purified DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

recommended

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Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - STOT SE 3

Target Organs

Respiratory system

Supplementary Hazards

EUH032

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Attempts to Construct an Enlarged pUC19 via Insertion of HindIII-digested Coliphage ? DNA
Muhamed Amirie
Journal of Experimental Microbiology and Immunology, 18, 96-101 (2014)
K Michael Lee et al.
Yeast (Chichester, England), 22(6), 431-440 (2005-04-26)
The Saccharomyces cerevisiae ADH2 promoter (P(ADH2)) is repressed several hundred-fold in the presence of glucose; transcription is initiated once the glucose in the medium is exhausted. The promoter can thus be utilized for effective regulation of recombinant gene expression in
Prolactin inhibits a major tumor-suppressive function of wild type BRCA1.
Chen KH and Walker Am
Cancer Letters, 375(2), 293-302 (2016)
Vernon K Ward et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(26), 11050-11055 (2007-06-22)
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Charles S Cockell et al.
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An epilithic microbial community was launched into low Earth orbit, and exposed to conditions in outer space for 548 days on the European Space Agency EXPOSE-E facility outside the International Space Station. The natural phototroph biofilm was augmented with akinetes
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Protocols

GenElute™ Gel Extraction procedure

The GenElute Gel Extraction kit is designed for the rapid purification of 50 bp to 10 kb linear DNA fragments and plasmids from standard or low-melting agarose gels.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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