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MAK086

Sigma-Aldrich

Acetate Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

General description

Acetate, the ionized form of acetic acid, is a critical metabolite in fatty acid and carbohydrate metabolism. The majority of acetate is utilized as the conjugate to coenzyme A (CoA), acetyl-CoA. Acetyl-CoA is a precursor to fatty acid and cholesterol synthesis and an important starting compound for the citric acid cycle. Acetyl-CoA also serves as a precursor for the synthesis of acetylcholine and as the donor for acetyl groups for post-translational acetylation reactions of histone and non-histone proteins. Acetate is generated from the metabolism of carbohydrates, amino acids, fatty acids, and ethanol.

The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.

Application

Acetate colorimetric assay kit has been used to determine acetate concentrations calorimetrically.

Suitability

Suitable for the detection of acetate in serum, plasma, animal tissues (liver, kidney, muscle, heart etc.), cell culture (adherent or suspension cells) and food.

Principle

The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.

replaced by

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

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Acetyl Coenzyme A: A Central Metabolite and Second Messenger
Federico P, et al.
Cell Metabolism, 21(6), 805-821 (2015)
Acetyl-CoA the key factor for survival or death of cholinergic neurons in course of neurodegenerative diseases.
Szutowicz A, et al.
Neurochemical Research, 38(8), 1523-1542 (2013)
A Pichia anomala Strain (P. anomala M1) Isolated from Traditional Greek Sausage is an Effective Producer of Extracellular Lipolytic Enzyme in Submerged Fermentation
Maria P and Emmanuel M P
Fermentation, 3(3), 43-43 (2017)
A refined genome-scale reconstruction of Chlamydomonas metabolism provides a platform for systems-level analyses.
Imam S, et al.
The Plant Journal, 84(6), 1239-1256 (2015)
Sheng Li et al.
Bioresource technology, 239, 285-293 (2017-05-23)
This study investigated the impact of reverse salt flux (RSF) on microbe community and bio-methane production in a simulated fertilizer driven FO-AnMBR system using KCl, KNO

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