MAK044
Free Fatty Acid Quantitation Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Free Fatty Acid Assay Kit
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About This Item
UNSPSC Code:
12161503
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
General description
Free fatty acids, also know as nonesterified fatty acids, circulate in the plasma bound to albumin. The majority of free fatty acids are derived from either dietary sources or are mobilized from adipose tissue. Plasma free fatty acids are elevated in many obesity-related disorders and may contribute to insulin resistance in peripheral tissues. Conditions such as sepsis and tumors producing lipoactive homones may also be associated with elevated free fatty acid levels.
Abnormalities in fatty acid metabolism causes fatty liver disease. Accumulation of free fatty acid in myocytes leads to the risk of cardiac dysfunction. Increased fatty acid β-oxidation is implicated in cardiac ischemia.
Abnormalities in fatty acid metabolism causes fatty liver disease. Accumulation of free fatty acid in myocytes leads to the risk of cardiac dysfunction. Increased fatty acid β-oxidation is implicated in cardiac ischemia.
Application
Free Fatty Acid Quantitation Kit has been used in the following:
- to measure lipid hydrolysis, by determining the free fatty acid in digested sample.
- to measure plasma and serum free fatty acids level.
- to quantify free fatty acids level in liver tissue.
Suitability
Suitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc.
Principle
In this kit, the concentration of fatty acids (C8 and longer) is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the fatty acids present.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Certificates of Analysis (COA)
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Transgenic expression of fatty acid transport protein 1 in the heart causes lipotoxic cardiomyopathy.
Chiu H C, et al.
Circulation Research, 96(2), 225-233 (2005)
Sarmistha Mukherjee et al.
Hepatology (Baltimore, Md.), 65(2), 616-630 (2016-11-04)
The regenerative capacity of the liver is essential for recovery from surgical resection or injuries induced by trauma or toxins. During liver regeneration, the concentration of nicotinamide adenine dinucleotide (NAD) falls, at least in part due to metabolic competition for
Kuifen Ma et al.
Iranian journal of basic medical sciences, 20(11), 1207-1212 (2018-01-05)
Arachidonic Acid/5-lipoxygenase (AA/5-LOX) pathway connects lipid metabolism and proinflammatory cytokine, which are both related to the development and progression of nonalcoholic fatty liver disease (NAFLD). Therefore, the present study was designed to investigate the role of AA/5-LOX pathway in progression
Lipid metabolism and liver inflammation. II. Fatty liver disease and fatty acid oxidation
Reddy J K and Sambasiva R M
American Journal of Physiology: Gastrointestinal and Liver Physiology, 290(5) (2006)
Association of Temperament and acute stress responsiveness with Productivity, Feed efficiency, and Methane emissions in Beef cattle: an Observational study
Llonch P, et al.
Frontiers in veterinary science, 3, 43-43 (2016)
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