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M6036

Sigma-Aldrich

Myoglobin from human heart

≥95% (SDS-PAGE), buffered aqueous glycerol solution

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

human heart

Quality Level

Assay

≥95% (SDS-PAGE)

form

buffered aqueous glycerol solution

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... MB(4151)

General description

Myoglobin is a heme-related, low-molecular-weight protein. It is mainly found in cardiac and skeletal muscle.

Application

Myoglobin from human heart has been used as a negative control in monitoring isoAsp formation during storage. It has also been used as an antigen in the study to identify self-antigens recognized by serum autoantibodies from unimmunized mice strains.
Myoglobin from human heart was used to study the interactions of a platinum (II) 9-aminoacridine complex with proteins and oligonucleotides by ESI-TOF MS. It was also used in the evaluation of plasma myoglobin using the Stratus CS fluorometer.

Biochem/physiol Actions

Myoglobin may help to increase diffusion and also stores O2 in oxidative muscle. It can momentarily act as an oxygen reservoir to supply oxygen when there is inadequate blood oxygen delivery at the time of intense muscular activity.

Physical form

Solution in 20 mM Tris-HCl, 1 mM EDTA and 50% glycerol, pH 8.5, 2 mg protein per mL (Lowry)

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Signe Helbo et al.
PloS one, 9(5), e97012-e97012 (2014-06-01)
The discovery that cysteine (Cys) S-nitrosation of trout myoglobin (Mb) increases heme O2 affinity has revealed a novel allosteric effect that may promote hypoxia-induced nitric oxide (NO) delivery in the trout heart and improve myocardial efficiency. To better understand this
P Couck et al.
Acta clinica Belgica, 60(2), 75-78 (2005-08-09)
Circulating myoglobin is recognized as an early and sensitive marker of acute coronary diseases. Long turnaround time of myoglobin assays jeopardize their clinical utility. We evaluated the analytical performance of the Stratus CS fluorometric enzyme immunoassay based on dendrimer technology
Iron metabolism and its disorders
Clinical biochemistry of domestic animals, 259-285 (2008)
A High-Throughput Bioluminescent Assay to Monitor the Deamidation of Asparagine and Isomerization of Aspartate Residues in Therapeutic Proteins and Antibodies
Hsiao K, et al.
Journal of Pharmaceutical Sciences, 106(6), 1528-1537 (2017)
Oxygen and Carbon Dioxide Transport
Quantitative Human Physiology (2017)

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