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L3397

Sigma-Aldrich

LB Broth (Luria low salt)

Powder microbial growth medium

Synonym(s):

Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.85

grade

for molecular biology

Quality Level

200

sterility

non-sterile

form

powder

composition

NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(1.55% solution)

suitability

suitable for molecular biology
nonselective for Escherichia coli
nonselective for coliforms

application(s)

food and beverages
microbiology

storage temp.

room temp

General description

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Application

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Features and Benefits

Luria′s low salt LB powder provides:
  • Standard formulation
  • A budget-friendly alternative to liquid
  • Easy scale-up using larger package sizes

Preparation Note

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Reconstitution

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Difco Manual, 272-272 (1998)
Giorgia Manzo et al.
ACS infectious diseases, 7(8), 2310-2323 (2021-07-31)
Reliable antimicrobial susceptibility testing is essential in informing both clinical antibiotic therapy decisions and the development of new antibiotics. Mammalian cell culture media have been proposed as an alternative to bacteriological media, potentially representing some critical aspects of the infection
Michel Godin et al.
Nature methods, 7(5), 387-390 (2010-04-13)
We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in
Alex Golberg et al.
AAPS PharmSciTech, 10(3), 881-886 (2009-07-03)
The purpose of this study was to examine the feasibility of using irreversible electroporation (IRE) as a non-chemical method for eliminating microorganisms of liquid drugs. The studied drug was a topical ophthalmic medication, a pharmaceutical field in which the problem
Atlas, R. M., L.C. Parks
Handbook of Microbiological Media (1993)
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Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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