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Key Documents

GE28-4064-09

Sigma-Aldrich

Tricorn 5/50 Column

Synonym(s):

5/50 Tricorn Column, Tricorn Column

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About This Item

UNSPSC Code:
41121800
NACRES:
SB.52

technique(s)

LPLC: suitable

General description

Tricorn 5 and 10 Coarse Filter Kit is used for large volume/repeated loading of clarified sample to reduce the risk of clogging in combination with capture media. Both filter and coarse filter kits include top and bottom adapters, bottom filters, and EPDM O-rings, five of each.
Tricorn Packing Equipment includes the Packing Equipment 5/50 (5-mm connector and 50-mm glass tube) or 10/100 (10-mm connector and 100-mm glass tube), EPDM O-rings and bottom unit, cap, and stop plug.

Features and Benefits

Broad range of column dimensions suitable for many different purification needs.
Glass tubes coated with plastic film prevent harm if the column is dropped or operated outside its specification.
Locking ring prevents accidental compression of media bed.
Separate packing connector allows simple column packing for a broad range of media.
The modern integrated, snap-on design results in a column that is easy to assembly, operate, and maintain.
Tricorn empty columns are designed for high performance separations on small and medium scale. Pressure stability up to 100 bar and durable column material ensure reliable and reproducible operation as well as compatibility with the majority of the commercial lab media.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Polishing of MAbs Using Capto Adhere ImpRes in Bind/Elute Mode

In these studies, the binding capacity for MAbs and the efficiency in the clearance of impurities using Capto adhere ImpRes in bind/elute mode was evaluated.

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins

This page describes efficient column packing and preparation for affinity chromatography of GST-tagged proteins using GE Healthcare products.

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.

Column Packing and Preparation

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using GE Healthcare products.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

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