GE28-4017-48
GSTrap™ 4B
Cytiva 28-4017-48, pack of 5 × 5 mL
Synonym(s):
GSTrap Column
About This Item
Recommended Products
ligand
glutathione
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 28-4017-48
parameter
<20 mL/min flow rate
42 psi (H2O at 25°C)
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
4-13
working range
4-13
capacity
>25 mg binding capacity (horse liver GST/ml medium)
General description
Application
Features and Benefits
- High binding capacity.
- Mild elution conditions preserving Protein antigenicity and function.
- Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
- GSTrap™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA® design.
Storage and Stability
Analysis Note
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.
Protocols
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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