GE17-5474-02
MabSelect SuRe™ LX
Cytiva 17-5474-02, pack of 200 mL
Synonym(s):
Protein purification resin
About This Item
Recommended Products
ligand
alkali-stabilized protein A-derived (E. coli)
packaging
pack of 200 mL
manufacturer/tradename
Cytiva 17-5474-02
storage condition
(20% Ehtanol)
matrix
highly cross-linked agarose
avg. part. size
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning in place
2-13.7
working range
3-12
capacity
~60 mg binding capacity
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
MabSelect™ SuRe™ LX is a member of the MabSelect™ family of affinity chromatography media for the capture of monoclonal antibodies (MAbs) at process scale. MabSelect™ SuRe™ LX shares features and benefits of MabSelect™ and MabSelect™ SuRe™ such as a rigid, high-flow agarose matrix that ensures excellent pressure/flow properties, low non-specific binding that leads to low impurity levels in the eluate pool, and an oriented ligand coupling for optimal binding capacity. It also features the same alkali-tolerant rProtein A ligand as MabSelect™ SuRe. This ligand provides greater stability than conventional protein A-based media in the alkaline conditions used in cleaning-in-place (CIP) and sanitization protocols. The enhanced alkali stability of MabSelect™ SuRe™ improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl. Process economy is also improved by the increased dynamic binding capacity of MabSelect™ SuRe™ LX.
Just as for MabSelect™ SuRe, the novel ligand construct of MabSelect™ SuRe™ LX has been proven to have an increased stability towards proteases. Therefore, the level of leakage of the MabSelect™ SuRe™ LX ligand during elution is low.
MabSelect™ SuRe™ has been demonstrated to give generic elution conditions for different monoclonal antibodies which can be applied also to MabSelect™ SURe LX since it utilizes the same ligand. This is an advantage when designing generic purification platform processes. The feature is also correlated to the construction of the MabSelect™ SuRe™ ligand.
MabSelect™ SuRe™ LX is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect™ SuRe™ meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Novel, alkali-tolerant rProtein A ligand withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
- Reduces the risk of product contamination and carryover
- Improves performance and reduces overall costs
- High dynamic binding capacity (DBC) for high titer cultures reduces process time and amount of medium used
- Generic elution profile for different monoclonal antibodies enables platform approach to purification
- High-flow agarose matrix allows the processing of large feed volumes
Storage and Stability
Analysis Note
Legal Information
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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