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GE17-5247-01

HisTrap High Performance

Cytiva 17-5247-01, pack of 5 × 1 mL

Synonym(s):

Nickel affinity chromatography

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

form

prepacked column

crosslinking

cross-linked

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5247-01

storage condition

(20% Ethanol)

parameter

4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)

technique(s)

liquid chromatography (LC): suitable

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

matrix active group

Nickel

avg. part. size

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

≥40 mg binding capacity (histidine-tagged protein/ml medium)(H20 at room temperature)
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

separation technique

affinity

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General description

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

HisTrap HP 1 mL and 5 mL columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be copurified with the tagged target protein.

The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

For convenient scaling up of histidine-tagged protein purification, use 20 mL HisPrep FF 16/10 columns. Ni Sepharose 6 Fast Flow, the medium prepacked in HisTrap FF and HisPrep FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.

Features and Benefits

  • High binding capacity, at least 40 mg/mL chromatography medium.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Environment

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from GE Healthcare.

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

Protocols

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from GE Healthcare.

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

This page shows how to purify protein A-tagged proteins using GE Healthcare products.

How to isolate proteins from inclusion bodies using GE Healthcare products.

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