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G8511

Supelco

β-Galactosidase from Escherichia coli

For use as a marker in SDS-PAGE

Synonym(s):

β-D-Galactoside galactohydrolase, Lactase

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About This Item

CAS Number:
9031-11-2
Enzyme Commission number:
3.2.1.23 (BRENDA, IUBMB)
EC Number:
232-864-1
MDL number:
MFCD00130623
UNSPSC Code:
12352200
NACRES:
NA.25

mol wt

~116 kDa

packaging

vial of 0.5 mg

storage temp.

2-8°C

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Application

β-Galactosidase from Escherichia coli has been used as an internal standard in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It has been used in the protein ladder mix for optimization of microfluidic western blotting assay.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Microfluidic Western blotting of low-molecular-mass proteins.
Gerver RE and Herr AE
Analytical Chemistry, 86, 10625-10632 (2014)
Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.
Tomaszewska-Gras J, et al.
Meat Science, 62, 61-66 (2002)
Screening of high-risk Gaucher disease patients using dried blood spots techniques.
Filippo Vairo et al.
Gene, 523(1), 114-115 (2013-04-10)
Natalya Chernichenko et al.
JAMA otolaryngology-- head & neck surgery, 139(2), 173-182 (2013-02-23)
To examine the therapeutic effects of an attenuated, replication-competent vaccinia virus (GLV-1h68) against a panel of 5 human salivary gland carcinoma cell lines. The susceptibility of 5 salivary gland carcinoma cell lines to infection and oncolysis by GLV-1h68 was assessed
Scott W McPherson et al.
Journal of immunology (Baltimore, Md. : 1950), 190(10), 4971-4981 (2013-04-16)
Extrathymically derived regulatory T cells (iTregs) protect against autoimmunity to tissue-specific Ags. However, whether Ag-specific iTreg generation and function is limited to secondary lymphoid tissue or whether it can occur within the tissue-specific local environment of the cognate Ag remains
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For use as a marker in SDS-PAGE; lactate dehydrogenase; contaminant; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

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