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G7765

Sigma-Aldrich

Gelatin from cold water fish skin

40-50% in H2O

Synonym(s):

Gelatine, Teleostean gelatin

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About This Item

CAS Number:
9000-70-8
MDL number:
MFCD00081638
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

fish skin

Quality Level

200

form

liquid

mol wt

~60 kDa

contains

0.15% propyl p-hydroxybenzoate as preservative
0.2% methyl p-hydroxybenzoate as preservative

concentration

40-50% in H2O

technique(s)

ELISA: suitable
cell culture | mammalian: suitable
immunocytochemistry: suitable
western blot: suitable

storage temp.

2-8°C

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General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water.
Gelatin is derived from collagen and is the most abundant protein in the extracellular matrix (ECM) of the tissues. It is a bioactive material. Gelatin consists of matrix metalloproteinase (MMP) sensitive protein sequences.

Application

Gelatin from cold water fish skin has been used in an incubation medium (IM) in immunolabelling of rat brain sections, as a component of rinse buffer in western blotting of various cells, as a blocking agent in immunofluorescent staining of HeLa cells.
Used as a blocking agent in immunochemistry.

Biochem/physiol Actions

Gelatin is cross-linked either chemically or enzymatically to form a three dimensional (3D) hydrogel.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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O.J. Day and H.G. Plascencia GonzAlez
Aquaculture (Amsterdam, Netherlands), 4, 221-228 (2000)
Heterogeneous expression of poliovirus receptor-related proteins in human cells and tissues.
Freistadt M S, et al.
Molecular and Cellular Biology, 10(11), 5700-5706 (1990)
OGFOD1, a novel modulator of eukaryotic translation initiation factor 2alpha phosphorylation and the cellular response to stress
Wehner K A, et al.
Molecular and Cellular Biology, 30(8), 2006-2016 (2010)
Johan Lindgren et al.
Scientific reports, 7(1), 13324-13324 (2017-10-19)
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