FD10S
Fluorescein isothiocyanate–dextran
average mol wt 10,000
Synonym(s):
FITC–Dextran
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About This Item
Recommended Products
biological source
sugar beets root
Quality Level
conjugate
FITC conjugate
form
powder
mol wt
average mol wt 10,000
extent of labeling
0.003-0.020 mol FITC per mol glucose
color
yellow to yellow-orange
solubility
water: 25 mg/mL, cloudy to hazy, yellow to orange
storage temp.
2-8°C
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Application
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
Preparation Note
Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.
Other Notes
Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Matthias Vonbrüll et al.
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Antonini, E. et al.
Biopolymers null
The structure of NRRL B-512F dextran, methylation studies.
Van Cleve JW, Schacfer WC and Rist CE
Journal of the American Chemical Society, 78(17), 4435-4438 (1956)
Clotilde Cadart et al.
Nature communications, 9(1), 3275-3275 (2018-08-18)
Despite decades of research, how mammalian cell size is controlled remains unclear because of the difficulty of directly measuring growth at the single-cell level. Here we report direct measurements of single-cell volumes over entire cell cycles on various mammalian cell
Evaluation of the Periodate Oxidation Method for Structural Analysis of Dextrans.
John C. Rankin , Allene Jeanes
Journal of the American Chemical Society, 17, 4435-4441 (1954)
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