F0500

Fast Blue RR Salt

crystalline

• Synonym(s): 4-Benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt, Azoic Diazo No. 24
• Linear Formula: C₁₅H₁₄ClN₃O₃ · 1/2 ZnCl₂
• CAS Number: 14726-29-5
• Molecular Weight: 387.89
• Colour Index Number: 37155
• EC Number: 238-777-5
• MDL number: MFCD00074762
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894722
• NACRES: NA.47

Properties

• form: crystalline
• Quality Level: 200
• solubility: water: 1 mg/mL, clear to hazy, yellow-green
• ε (extinction coefficient): ≥15000 at 326-332 nm; ≥7000 at 390-396 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: [Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4
• InChI: 1S/2C15H13N3O3.4ClH.Zn/c2*1-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2
• InChI key: LLARVKAOQLUCQI-UHFFFAOYSA-L

Description

Application

Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appears black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves