Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

D2158

Sigma-Aldrich

Histodenz™

nonionic density gradient medium

Synonym(s):

5-(N-2,3-Dihydroxypropylacetamido)-2,4,6-triiodo-N,N′-bis(2,3-dihydroxypropyl)isophthalamide

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Empirical Formula (Hill Notation):
C19H26I3N3O9
CAS Number:
66108-95-0
Molecular Weight:
821.14
Beilstein:
2406632
EC Number:
266-164-2
MDL number:
MFCD00077732
UNSPSC Code:
12352207
PubChem Substance ID:
24893646
NACRES:
NA.75

Quality Level

200

form

powder

application(s)

hematology
histology

storage temp.

room temp

SMILES string

CC(=O)N(CC(O)CO)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I

InChI

1S/C19H26I3N3O9/c1-8(29)25(4-11(32)7-28)17-15(21)12(18(33)23-2-9(30)5-26)14(20)13(16(17)22)19(34)24-3-10(31)6-27/h9-11,26-28,30-32H,2-7H2,1H3,(H,23,33)(H,24,34)

InChI key

NTHXOOBQLCIOLC-UHFFFAOYSA-N

General description

HistoDenz™, also known as iohexol, is a non-ionic X-ray contrast compound developed specifically as an iodinated density gradient medium.

Application

HistoDenz™ is suitable for separating everything from macromolecules to viable cells by either rate-zonal or isopycnic centrifugation.
  • It has been used in the isolation and culturing of hepatic stellate cells.
  • It has also been employed in the extraction of microbial cells from environmental samples.
  • It has been used in LacZ (β-galactosidase) staining.
  • It has also been used for the enrichment of gonocytes.

Features and Benefits

HistoDenz™ offers several favorable properties as a gradient medium.
  • It has very low toxicity, low osmolality, high density, and low viscosity (compared to sucrose).
  • It is water-soluble, non-ionic, and chemically near-inert.

Legal Information

Histodenz is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

Iodixanol European Pharmacopoeia (EP) Reference Standard

Y0001294

Iodixanol

Extraction of Microbial Cells from Environmental Samples for FISH Approaches
Jennifer Pratscher
Methods in Molecular Biology, 2246 (2021)
Attachment of Pseudomonas putida onto differently structured kaolinite minerals: A combined ATR-FTIR and 1H NMR study.
Vasiliadou I A, et al.
Colloids and Surfaces. B, Biointerfaces, 84(2), 354-359 (2011)
Malin Helenius et al.
Scandinavian journal of urology, 49(5), 377-381 (2015-03-21)
Computed tomography urography (CTU) can be used to direct further investigation of patients if the bladder tumour detection rate is high. The aim of this study was to compare a CTU protocol including an enhancement-triggered scan and flexible cystoscopy for
Skeletal muscle tissue clearing for lacz and fluorescent reporters, and immunofluorescence staining.
Verma M, et al.
Methods in Molecular Biology, 129-140 (2016)
Optimization of culture conditions for short-term maintenance, proliferation, and colony formation of porcine gonocytes.
Awang-Junaidi, et al.
Journal of Animal Science and Biotechnology, 9(1), 8-8 (2018)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service