CMC0015
BL21(DE3) pLysE Chemically Competent Cells
Escherichia coli, rod shaped
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About This Item
UNSPSC Code:
41106202
NACRES:
NA.85
product name
BL21(DE3) pLysE Chemically Competent Cells, for protein expression
biological source
Escherichia coli
grade
for molecular biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
shipped in
dry ice
storage temp.
−70°C
Related Categories
General description
The BL21(DE3) pLysE Chemically Competent Cells produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products.
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Features and Benefits
- a transformation efficiency of >1 × 107 cfu/μg.
- economical prices
- offering superior value for everyday protein expression work.
Components
- BL21(DE3) pLysE chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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