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biological source
human prostate
growth mode
Adherent
karyotype
46XY
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human foreskin, fibroblast
Cell Line Description
Hs27 is one of a series of human foreskin fibroblast lines developed at NBL, California. The material was obtained from a normal new-born, and the cells possess the G6PD type A phenotype. Longevity studies carried out at NBL demonstrated that the cells can be propagated for 42 passages. There were no consistent aberrations in analysed cells. Ethnicity: Black.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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