1BR.3.N

90020508, human skin, Fibroblast

• UNSPSC Code: 41106514

Properties

• product name: 1BR.3.N, 90020508
• biological source: human skin
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human skin fibroblast, transformed

Cell Line Description

Derived from a normal fibroblast (1BR3 Sigma Catalogue number. 90011801) transformed with the plasmid pSV2neo. The genetic identity of 1BR3 (90011801), 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.

Application

DNA repair and cell growth studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,13
D13S317: 9,12
D16S539: 12,14
D5S818: 12
D7S820: 8,10
THO1: 9.3
TPOX: 11
vWA: 14,15

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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