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biological source
Rat x Rat Hybridoma
ImmunoDon
DA rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-L3T4, mouse
technique(s)
cell culture | mammalian: suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−196°C
Cell Line Description
The antibody is directed against the A epitope of the mouse L3T4 T cell antigen, the murine equivalent of CD4. Has been used in in vivo depleting and flow cytometry studies. Cambridge Enterprise Reference number AB16: www.enterprise.cam.ac.uk.
Immunogen
CBA mouse thymocytes
Culture Medium
RPMI 1640 + 2 mM glutamine + 0.05 mM 2-mercaptoethanol (2ME) + 20% Fetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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