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Key Documents

C-24310

Sigma-Aldrich

Melanocyte Growth Medium M3

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Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.81

technique(s)

cell culture | mammalian: suitable

Application

Melanocyte Growth Medium (Ready-to-use)

Features and Benefits

  • Highest degree of standardization achieved by BPE-free and serum-free formulation
  • No adverse effects of PMA (Phorbol Myristate Acetate) on experimental settings
  • Each produced media lot is tested for growth promoting activity and characteristic dendritic cell morphology
  • Compatible with all available PromoCell Melanocytes (NHEM)

Components

1x Basal Medium, 500ml
1x SupplementMix for 500ml

Quality

All lots of PromoCell Melanocyte Growth Medium M3 are subjected to comprehensive quality control tests using primary human melanocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested melanocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Warning

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Other Notes

Melanocyte Growth Medium M3 is designed for the isolation and cultivation of human melanocytes from human juvenile and human adult skin in a BPE- and serum-free environment. The medium does not contain PMA (Phorbol Myristate Acetate), which could interfere with some experimental settings. Cells cultured in this medium can be plated directly on tissue culture plastic and no matrix coating is needed. Isolated human juvenile and adult melanocytes show their characteristic dendritic morphology when cultured in this advanced cell culture medium.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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