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Key Documents

B8270

Sigma-Aldrich

Anti-Bovine Serum antibody produced in rabbit

whole antiserum

Synonym(s):

Anti-Serum, bovine

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About This Item

MDL number:
MFCD00162174
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

bovine

technique(s)

immunoelectrophoresis: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... Alb(280717)

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General description

Bovine serum is a component extracted from bovine blood. It serves as a nutrient supplement in various in vitro cell cultures. It is also used in the culture of cumulus-oocyte complexes. Anti-bovine serum antibody can be used in differential staining to estimate inner cell mass and trophectoderm cell count. Rabbit anti- bovine serum antibody reacts specifically with normal bovine serum.

Immunogen

Pooled normal bovine serum.

Application

Anti-Bovine Serum antibody produced in rabbit has been used as a supplement in Dulbecco′s modified eagle medium for culturing inner cell mass (ICM) and cohesive trophectoderm (TE).
Anti-bovine serum antibody (10% (v/v) in Hepes-SOF) has been used in immunosurgery and immunoelectrophoresis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Transcriptome profiling of bovine inner cell mass and trophectoderm derived from in vivo generated blastocysts
Hosseini, S. M. and others
BMC Developmental Biology, 15(1), 49-49 (2015)
S M Hosseini et al.
BMC developmental biology, 15, 49-49 (2015-12-19)
This study describes the generation and analysis of the transcriptional profile of bovine inner cell mass (ICM) and trophectoderm (TE), obtained from in vivo developed embryos by using a bovine-embryo specific array (EmbryoGENE) containing 37,238 probes. A total of 4,689
P Collas et al.
Molecular reproduction and development, 38(3), 264-267 (1994-07-01)
The developmental potential of bovine inner cell mass (ICM) and somatic differentiated (granulosa cell) nuclei was investigated using nuclear transplantation. ICM blastomeres were isolated after immunosurgery of day 7 in vitro produced blastocysts and cumulus granulosa cells recovered from in
The Effect of Cryopreservation on the Mouse Embryos at Various-pronuclear Stages.
M. C. Park
Journal of Animal Science, 22(2), 174 -1180 (2009)
M Sims et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(13), 6143-6147 (1994-06-21)
We report here the isolation and in vitro culture of bovine inner cell mass (ICM) cells and the use of ICM cells in nuclear transfer to produce totipotent blastocysts that resulted in calves born. Of 15 cell lines represented in
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