B0631

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt

≥90%

• Synonym(s): 5-BrdUTP
• Empirical Formula (Hill Notation): C₉H₁₄BrN₂O₁₄P₃
• CAS Number: 102212-99-7
• Molecular Weight: 547.04
• MDL number: MFCD00057405
• UNSPSC Code: 41106305
• PubChem Substance ID: 24891527
• NACRES: NA.51

Properties

• biological source: synthetic (organic)
• Quality Level: 200
• Assay: ≥90%
• form: powder
• solubility: water: 50 mg/mL, clear, colorless to faintly yellow
• storage temp.: −20°C
• SMILES string: [Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H14BrN2O14P3.Na.H/c10-4-2-12(9(15)11-8(4)14)7-1-5(13)6(24-7)3-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h2,5-7,13H,1,3H2,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
• InChI key: UWZGQOWZUSBZMC-UHFFFAOYSA-N

Description

General description

5-Bromo-2′-deoxyuridine 5′-triphosphate is used for incorporation into DNA for studying normal and tumor cell proliferation profiles.

Application

5-Bromo-2′-deoxyuridine 5′-triphosphate (5-BrdUTP) may be used as a labeling TdT substrate for terminal deoxynucleotidyl transferase (TdT) reactions or for DNA synthesis. BrdUTP is also used as an effective mutagenic agent. 5-BrdUTP labeling may be detected by immunological techniques or 5-BrdUTP may be derivitized by biotinylation or other methods for detection by fluorogenic or chromogenic methods.

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt has been used:

• in the labelling of 3′-OH termini of fragmented DNA with double stranded breaks in african green monkey kidney cells
• to label cleaved DNA ends in apoptosis assay in avian tissue sections
• for incorporation into mice prostates for immunohistochemistry studies

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves