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A6050

Sigma-Aldrich

Acrylamide/Bis-acrylamide

BioReagent, for molecular biology, 37:1 (ratio)

Synonym(s):

Acrylamide/Bisacrylamide, 37:1 (2.6% crosslinker), Polyacrylamide solution

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About This Item

MDL number:
MFCD00080848
UNSPSC Code:
41105319
PubChem Substance ID:
329770882
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

product line

BioReagent

form

powder

feed ratio

37:1 (ratio)

foreign activity

DNase, RNase and protease, none detected

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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General description

Acrylamide (AA) is a neurotoxin and carcinogen. It was found in potato products like, french fries and potato chips and also in several cereal-based products. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.

Application

Acrylamide/Bis-acrylamide has been used in the preparation of polyacrylamide gels for electrophoresis.
Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Reconstitution

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.
To make up 100 ml of A6050, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Use of potato flour in bread and flat bread
Flour and Breads and Their Fortification in Health and Disease Prevention, 247-259 (2011)
Genetic diversity analysis of Rhodothermus reflects geographical origin of the isolates
Petursdottir SK, et al.
Extremophiles : Life Under Extreme Conditions, 4(5), 267-274 (2000)
Acrylamide: a common food toxin related to physiological functions and health
Semla M, et al.
Physiological Research, 66(2), 205-205 (2017)

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