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A1602

Sigma-Aldrich

Amyloglucosidase from Aspergillus niger

ammonium sulfate suspension, ≥40 units/mg protein

Synonym(s):

1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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About This Item

CAS Number:
9032-08-0
Enzyme Commission number:
3.2.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00081350
UNSPSC Code:
12352204
NACRES:
NA.54

form

ammonium sulfate suspension

Quality Level

200

specific activity

≥40 units/mg protein

shipped in

wet ice

storage temp.

2-8°C

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General description

Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.

Application

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .
Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

Unit Definition

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

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