79636
Atto 590 NHS ester
suitable for fluorescence, BioReagent, ≥90.0% (degree of coupling)
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About This Item
Empirical Formula (Hill Notation):
C41H42ClN3O11
CAS Number:
Molecular Weight:
788.24
MDL number:
UNSPSC Code:
12352108
NACRES:
NA.32
Recommended Products
product line
BioReagent
Quality Level
Assay
≥90.0% (HPLC)
≥90.0% (degree of coupling)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
λ
in ethanol (with 0,1% trifluoroacetic acid)
UV absorption
λ: 597-663 nm Amax
suitability
suitable for fluorescence
detection method
fluorometric
storage temp.
−20°C
InChI
1S/C41H41N3O7.ClHO4/c1-9-42-31-18-33-29(16-26(31)22(3)20-40(42,5)6)37(30-17-27-23(4)21-41(7,8)43(10-2)32(27)19-34(30)50-33)28-15-24(11-12-25(28)38(47)48)39(49)51-44-35(45)13-14-36(44)46;2-1(3,4)5/h11-12,15-21H,9-10,13-14H2,1-8H3;(H,2,3,4,5)
InChI key
SHJDVERDESTYRQ-UHFFFAOYSA-N
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General description
Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes, which has an absorption maximum of 594 nm and an emission maximum of 624 nm. Atto 590-N-hydroxysuccinimide (NHS) is membrane permeable.
Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The Atto dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescence in situ hybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.
Application
Atto 590 NHS ester may be suitable for use in site-specific labeling of human embryonic kidney (HEK293T) cell lysates for western blotting, fluorescence, and widefield microscopy studies.
Molar absorption 120,000 1/M cm, abs: 593 nm, em: 620 nm, QY=0.93, Tfl 4.0 ns (unpublished data)
Features and Benefits
- Absorption maxima cover a range from 590 to 620 nm.
- Structural properties support extraordinary photostability.
- High fluorescence quantum yield and high thermal stability.
- The fluorescence characteristics of the dye are quite insensitive to environmental changes (e.g., pH changes). This holds for both excitation and emission wavelengths and for the decay time of their fluorescence emission.
- Compatible with common labeling procedures for proteins and amino-functionalized oligonucleotides.
Other Notes
Study of multistep energy transfer in a single photonic wire by FRET; employed in fluorescence energy transfer, FRET, scanning microscopy.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Y. Eberstein et al.
J. Chem. Phys., B 108, 93-93 (2004)
D Wildanger et al.
Journal of microscopy, 236(1), 35-43 (2009-09-24)
The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all-physics based
Mike Heilemann et al.
Journal of the American Chemical Society, 126(21), 6514-6515 (2004-05-27)
We demonstrate the synthesis and spectroscopic characterization of an unidirectional photonic wire based on four highly efficient fluorescence energy-transfer steps (FRET) between five spectrally different chromophores covalently attached to double-stranded DNA. The DNA-based modular conception enables the introduction of various
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Fluorescent Labeling of Peptides
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