32857

Wright Stain solution

according to Wright

• Synonym(s): Eosin Methylene blue solution according to Wright, Wright’s eosin methylene blue
• MDL number: MFCD00005038
• UNSPSC Code: 12171500
• PubChem Substance ID: 329754363
• NACRES: MA.02

Properties

• form: liquid
• Quality Level: 200
• quality: according to Wright
• impurities: methanol
• density: 0.8 g/cm³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
• InChI: 1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1
• InChI key: NXBZXYJXNIFCRJ-UHFFFAOYSA-M

Description

General description

Wright′s stain solution is commercially available and is generally used in hematology. The procedure of making it includes heating methylene blue with sodium bicarbonate, followed by combining the mixture with eosin. It is an alkaline solution; hence its concentration is continuously changing, owing to the progressive oxidizing action of the alkali, therefore Giemsa′s stain is added to preserve the azurophilic properties of the polychrome dyes.

Application

Wright stain solution was used during the exudate analysis of white blood cell concentration.

Safety Information

• Pictograms: GHS02,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H225 - H301 + H311 + H331 - H317 - H319 - H370
• Precautionary Statements: P210 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 51.8 °F
• Flash Point(C): 11 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves