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24365C

SAFC

EX-CELL® CD CHO Fusion

Animal-component free, chemically defined cell culture medium, without L-glutamine, without hypoxanthine, without sodium bicarbonate, without thymidine

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.71

Quality Level

product line

EX-CELL®

form

dry powder

technique(s)

cell culture | mammalian: suitable (suspension)

shipped in

ambient

storage temp.

2-8°C

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General description

EX-CELL® CD CHO FUSION is a chemically defined, animal-component free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells. The absence of any large macromolecules allows for isolation and purification of secreted proteins from the cells. This medium is supplied without L-glutamine to aid in media stability, to avoid L-glutamine degradation that causes ammonia build-up, and to provide an appropriate medium for the culture of CHO cells using the Glutamine Synthetase, or GS System. This medium does not contain hypoxanthine or thymidine to allow for its use with dihydrofolate reductase (DHFR) gene amplification systems.

Quantity

Formulated to contain 20.1 grams of powder per liter of medium.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
GS System is a trademark of Lonza Group Ltd.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Articles

This application note describes the benefits and performance of Cellvento® 4CHO-X Expansion Medium designed for multiple cell expansion steps from vial thaw to N-1 stages to generate higher biomass for inoculating fed-batch production bioreactors

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